Mechanistic studies to enable rational design of isobutylamine N-hydroxylase.

With the emergence of bacterial resistance, identification of new diseases, and the need for new therapeutics with different efficacies, our ability to design drugs to battle bacterial infections is becoming a more urgent priority. Natural products are often useful as therapeutics for humans, though problems such as side effects and production difficulties can preclude their successful development. This proposal seeks...

Enabling rational design of biodesulfurization: biochemical and structural characterization of dibenzothiophene monooxygenase

The long-term objective of this work is to enable effective engineering of the Rhodococcus erythropolis biodesulfurization pathway. We will begin our study of the catalytic mechanism of dibenzothiophene monooxygenase (DszC) by characterizing the functional roles of specific active site residues of DszC by site-directed mutagenesis, biochemical characterization, and X-ray c...

Engineering isobutylamine N-hydroxylase for applications in antibiotic biosynthesis

With the emergence of bacterial resistance, identification of new diseases, and the need for new therapeutics with different efficacies, our ability to design drugs to battle bacterial infections is becoming a more urgent priority. Natural products are often useful as therapeutics for humans, though problems such as side effects and production difficulties can preclude their successful development. This proposal s...